Ion Mobility for Metabolite Characterization: Improving Spectral Clarity of Data Independent Acquisitions

Library Number:
APNT135071703
Part Number:
720006919EN
Author(s):
Lisa Reid, Lauren Mullin, David Higton, Nathan Anderson
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
Related Products:
 
 

This data demonstrates the advantage of ion mobility separation combined with DIA for DMPK studies. Use of this orthogonal separation technique is capable of significantly improving the confidence and speed with which drug metabolism and degradation products can be identified. The dextromethorphan metabolite 2x + O product ion signals are relatively minor when compared to interfering matrix ions, these are not separated using traditional LC-MS analysis and therefore can complicate assignment. When this same data is analyzed incorporating the ion mobility dimension, matrix product ions are removed, and therefore, metabolite product ions are easily identified and confidently assigned.

 


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