An Automated Compliance-Ready LC-MS Workflow for Intact Mass Confirmation and Purity Analysis of Oligonucleotides

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Catalin Doneanu, Jonathan Fox, Emma Harry, Chris Knowles, Ying Qing Yu, Joseph Fredette, Weibin Chen
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Application Notes
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Application Notes
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This application note describes a streamlined, compliance-ready workflow for oligonucleotide analysis employing the BioAccord System operating under waters_connect HUB data acquisition and processing software.

  • A waters_connect workflow is shown to provide good mass accuracy (5–15 ppm) for intact mass confirmation of oligonucleotides of various lengths (10–35 mers), as well as phosphorothioated oligos. 
  • In addition, the automated processing method that was developed provides purity information for all the oligo sample components that are separated by ion-pair reversed-phase chromatography.
  • This automated, compliance-ready LC-MS workflow provides for a robust, easy-to-use solution for fast mass confirmation and purity analysis of oligonucleotides.

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