This application presents the validation of the quantitative MxP Quant 500 kit on a Waters Xevo TQ-XS Mass Spectrometer. The assay offers multiplexed MS/MS analysis of up to 630 metabolites and lipids from 26 analyte classes in only a 10-μL sample volume. In addition, 232 pre-defined metabolism indicators, that are biologically meaningful sums and ratios of metabolites and lipids, can be calculated by the BIOCRATES MetIDQ software tool, MetaboINDICATOR. Combined, these are 852 metabolic features that could be determined in one experiment with the MxP Quant 500 kit.
The data shown in this application note supports the high reliability and analytical potency of the MxP Quant 500 kit on the ACQUITY UPLC I-Class PLUS, Xevo TQ-XS system, delivering high level of global standardization in metabolomics with excellent lab-to-lab comparability.