In this application note we report a validated UPLC-MS/MS method based upon the Quick Polar Pesticides Method (QuPPE) and the novel use the Torus DEA Column (p/n 186007616) for the cost-effective determination of a range of polar pesticides, including glyphosate, in spinach. The ACQUITY UPLC I-Class System used with the Xevo TQ-XS mass spectrometer deliver the sensitivity to detect the pesticide residues at ppb levels.
The use of the Torus DEA Column represents a robust, cost-effective solution, providing excellent chromatographic performance for a wide range of anionic polar pesticides and metabolites. This is achieved in a single analysis, without being required to use derivatization, specialized equipment, or atypical mobile phases. This method can be easily implemented in routine testing laboratories as it is compatible with existing conventional HPLC and UPLC instrumentation. It has been demonstrated as suitable for checking compliance with EU MRLs and has the potential for screening at much lower concentrations, for example for food business operators’ due diligence testing.
Further details about using the Torus DEA Column for polar pesticide separations of glyphosate and its related compounds can be accessed in our method Start-up Guide.
*Please note: This Application Note was developed on a Torus DEA Column, improved performance can be achieved using the Waters Anionic Polar Pesticide Column coupled with our most recent Application Notes please contact Waters Chemistry Technical Services with any questions www.waters.com/contact.