Quantitative Analysis of THC and its Metabolites in Plasma Using Oasis PRiME HLB for Toxicology and Forensic Laboratories

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Xin Zhang, Jonathan P. Danaceau, and Erin E. Chambers
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Application Notes
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This application note details the extraction of THC-OH, THC-COOH, and THC from plasma samples using a novel SPE sorbent, Oasis PRiME HLB, in a μElution format for forensic toxicology applications.

The unique nature of this sorbent enabled the elimination of conditioning and equilibration steps, simplifying the extraction procedure, and speeding up the sample preparation workflow. In addition, the μElution format enabled the direct injection of extracts without evaporation or reconstitution, saving time and minimizing the risk of nonspecific binding. One key attribute of this sorbent is its ability to remove phospholipids resulting in a much cleaner final sample.

In conclusion, Oasis PRiME HLB has been successfully used to achieve consistent recoveries with low matrix effects as well as accurate quantification at 4 orders of magnitude from plasma samples.

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