Hydrophilic-Interaction Chromatography (HILIC) for LC-MS/MS Analysis of Monoamine Neurotransmitters using XBridge BEH Amide XP Columns

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Jonathan P. Danaceau, Kenneth J. Fountain, and Erin E. Chambers
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This work presents the application of HILIC for the analysis of monoamine neurotransmitters using an XBridge™ BEH Amide XP column. Development of the successful chromatographic method depends upon the systematic optimization of organic mobile phase ionic strength to obtain the best peak shape and sensitivity. Choice of stationary phase and column temperature are also important. The resulting method achieves baseline separation for the most polar and challenging analytes in a short analysis time without the use of ion-pairing reagents that are often necessary in reversed-phase analysis of these compounds.

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