LipidQuan-R for Diacyl-Phosphatidylcholines in Human Serum: A Rapid, Targeted UPLC-MS/MS Method for Lipidomic Research Studies

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Billy J. Molloy
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Targeted Metabolomics

A rapid, targeted UPLC-MS/MS methodology has been developed for the semi-quantitative analysis of 57 diacyl-phosphatidylcholine species in human serum. This research method has demonstrated to be suitable for the analysis of these analytes at physiologically relevant levels in human serum. This LipidQuan-R method is optimized on the same versatile LC-MS platform that has previously been utilized for the analysis of various classes of compounds including bile acids, amino acids, free fatty acids, tryptic peptides, acylcarnitines, tryptophan metabolites, and various lipids. This new method extends the LipidQuan-R/MetaboQuan-R suite of methodologies, and allows the analysis of an expanding set of metabolites and lipids, when operated sequentially in a targeted multi-omics workflow.

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