Triple Quadrupole Mass Spectrometry (Xevo TQ-XS) for the Quantification of Monoclonal Antibody Light Chains in Plasma
The work herein describes the development and optimization of sample preparation and LC-MS/MS methodology for the sensitive quantification of mAb subunit light chains using selective column chemistry and triple quadrupole mass spectrometry.
Combined with the identification of generic and sensitive MS/MS fragments, these methods enabled the high sensitivity, and accurate quantification of mAb subunit light chains via triple quadrupole mass spectrometry.
- Highly specific immunoaffinity capture techniques and a simple workflow for the partial reduction of monoclonal antibodies were developed and optimized
- BioResolve RP mAb Polyphenyl columns successfully resolved and enabled fast (8.5 minute cycle time) chromatographic separation of the mAb subunit light chains for both adalimumab and cetuximab
- Using only 10 μL of rat plasma, adalimumab subunit light chains can be quantified reliably, achieving LLOQs of 25 ng/mL and a linear dynamic range >3.5 orders of magnitude