Triple Quadrupole Mass Spectrometry (Xevo TQ-XS) for the Quantification of Monoclonal Antibody Light Chains in Plasma

Library Number:
APNT135040631
Part Number:
720006716EN
Author(s):
Caitlin M. Dunning, Mary Lame, and Mark Wrona
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
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The work herein describes the development and optimization of sample preparation and LC-MS/MS methodology for the sensitive quantification of mAb subunit light chains using selective column chemistry and triple quadrupole mass spectrometry.

Combined with the identification of generic and sensitive MS/MS fragments, these methods enabled the high sensitivity, and accurate quantification of mAb subunit light chains via triple quadrupole mass spectrometry.

  • Highly specific immunoaffinity capture techniques and a simple workflow for the partial reduction of monoclonal antibodies were developed and optimized
  • BioResolve RP mAb Polyphenyl columns successfully resolved and enabled fast (8.5 minute cycle time) chromatographic separation of the mAb subunit light chains for both adalimumab and cetuximab
  • Using only 10 μL of rat plasma, adalimumab subunit light chains can be quantified reliably, achieving LLOQs of 25 ng/mL and a linear dynamic range >3.5 orders of magnitude

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