Analysis of Plasma Proteins using 1 mm Scale Chromatography and the Xevo G2-XS QTof Operating in SONAR Acquisition Mode

Library Number:
APNT135009259
Part Number:
720006493EN
Author(s):
Christopher Hughes and Lee Gethings
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Technical Notes
Related Products:
 
 
 
 
ACQUITY UPLC I-Class System

This technology brief demonstrates the utility of a larger scale chromatography system for plasma proteomics over traditionally employed systems. In addition to increased throughput, this methodology would be aligned with other omics analytical scale methods, such as metabolomics and lipidomics.

This study shows the utility of 1 mm scale chromatography for the analysis of typical samples from plasma proteomic experiments, where sample amounts tend to be present at elevated levels. The gradient has been delivered using an ACQUITY UPLC I-Class System and therefore raises the prospect of a single LC-MS platform for proteomics and other omic applications, such as metabolomics and lipidomics. The SONAR DIA acquisition method offers a compatible acquisition speed for rapid chromatographic methods of this type.


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