This technology brief demonstrates the utility of a larger scale chromatography system for plasma proteomics over traditionally employed systems. In addition to increased throughput, this methodology would be aligned with other omics analytical scale methods, such as metabolomics and lipidomics.
This study shows the utility of 1 mm scale chromatography for the analysis of typical samples from plasma proteomic experiments, where sample amounts tend to be present at elevated levels. The gradient has been delivered using an ACQUITY UPLC I-Class System and therefore raises the prospect of a single LC-MS platform for proteomics and other omic applications, such as metabolomics and lipidomics. The SONAR DIA acquisition method offers a compatible acquisition speed for rapid chromatographic methods of this type.