This application note demonstrates a high-throughput UPLC-MS/MS research method for the semi-quantitative analysis of various tryptic peptides in human serum samples.
A rapid UPLC-MS/MS methodology has been developed for the research analysis of proteins. This method has been demonstrated to be suitable for the analysis of physiologically relevant levels of multiple proteins in human serum. This method utilizes a generic LC-MS platform that can be used for various compound classes (including metabolomics, lipidomics, and proteomics). Deployment of this method in conjunction with other complementary methods available on the Waters MetaboQuan™ website can form the basis of a comprehensive suite of targeted multi-omic workflows.