High Sensitivity Intact Monoclonal Antibody (mAb) HRMS Quantification

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Yun Wang Alelyunas, Henry Shion, Mark Wrona
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The complexity of trastuzumab, and biotherapeutic proteins in general, require a multifaceted and flexible approach for their characterization and quantification. Highly sensitive direct quantitation at the intact level was achieved using a Waters Vion IMS QTof and UNIFI.

The present data suggests that HRMS is capable of providing highly sensitive intact level quantification of biotherapeutic proteins. Data derived from the intact level analysis will complement those from ligand binding assays or LC/MS/MS based surrogate peptide approaches for a complete understanding of the proteins in question.

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