Accurate and Sensitive LC-MS/MS Quantification of Adalimumab in Serum/Plasma: Impact of Sample Preparation on Method Performance

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Caitlin Dunning, Mary Lame, Steven Calciano, and Kelly Doering
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Application Notes
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This application note describes the sensitive and selective quantification of adalimumab from serum/plasma using a kit-based approach for sample digestion and peptide level clean-up. Coupling different enrichment techniques to the standardized approach of ProteinWorks eXpress Digest and SPE Clean-up Kits provided substantial sensitivity benefit with the increased complexity of each technique.

Using a kit-based approach for the bioanalytical method development of adalimumab simplified and standardized the workflow and facilitated the accurate and robust quantification of adalimumab across multiple, complex sample preparation techniques. This approach enables traditional “small molecule” scientists to quickly and successfully develop LC-MS methods for protein quantification from complex biological matrices.

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