Comparison of Tandem and High Resolution Mass Spectrometry for the Quantification of the Monoclonal Antibody, Trastuzumab in Plasma

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Mary Lame, Caitlin Dunning, Yun Alelyunas, and Mark Wrona
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Application Notes
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Application Notes
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Protein Quantification

This technology brief demonstrates the sensitive and robust HRMS quantification of trastuzumab from plasma.

The Xevo TQ-XS Tandem Quadrupole is compared with Xevo G2-XS QTof for the bioanalytical quantification of trastuzumab prepared from plasma. For HRMS quantification, best sensitivity and performance was achieved using Tof-MRM mode. In addition, this performance was highly comparable to the Xevo TQ-XS Tandem Quadrupole MS results, achieving LLOQs within 2-fold and 4-orders of linearity. This highly reproducible data demonstrates that the Xevo G2-XS QTof System can be used to provide sensitive, accurate, and robust quantitative results.

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