Comparison of Tandem and High Resolution Mass Spectrometry for the Quantification of the Monoclonal Antibody, Trastuzumab in Plasma

Library Number:
APNT134970917
Part Number:
720006207EN
Author(s):
Mary Lame, Caitlin Dunning, Yun Alelyunas, and Mark Wrona
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
Related Products:
Protein Quantification
 
 
 
 

This technology brief demonstrates the sensitive and robust HRMS quantification of trastuzumab from plasma.

The Xevo TQ-XS Tandem Quadrupole is compared with Xevo G2-XS QTof for the bioanalytical quantification of trastuzumab prepared from plasma. For HRMS quantification, best sensitivity and performance was achieved using Tof-MRM mode. In addition, this performance was highly comparable to the Xevo TQ-XS Tandem Quadrupole MS results, achieving LLOQs within 2-fold and 4-orders of linearity. This highly reproducible data demonstrates that the Xevo G2-XS QTof System can be used to provide sensitive, accurate, and robust quantitative results.


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