A Generic Kit-Based Approach for LC-MS/MS Quantification of Urinary Albumin for Clinical Research

Library Number:
APNT134940500
Part Number:
720006034EN
Author(s):
Mary Lame, Caitlin Dunning, and Erin Chambers
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
Related Products:
 
 
 
Protein Quantification
 
 

This application note describes a high sensitivity LC-MS quantification of a protein biomarker.

LC-MS is widely accepted for protein quantification for use in clinical research. However, this workflow can be complex and time consuming, often taking 24 hours to achieve analytically sensitive and accurate quantification. Using ProteinWorks eXpress Digest and ProteinWorks μElution SPE Clean-Up Kits, accurate quantification over 3.5 orders of magnitude (0.1–500 μg/mL) was readily achieved. The digestion time in this work is only 2 hours, which is 9X faster than previously published LC-MS methodologies.3 The analytical sensitivity represents a 50X improvement over earlier LC-MS methods and accurately quantifies low endogenous levels in urine while approaching the analytical sensitivity of immunoassays.


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