A Rapid, Kit-based Approach for LC-MS/MS Quantification of Apolipoprotein A1 in Plasma

Library Number:
APNT134904184
Part Number:
720005777EN
Author(s):
Hua Yang, Mary Lame, Sherri Naughton, and Erin Chambers
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
Related Products:
ACQUITY UPLC System
 
ACQUITY UPLC System
 
ACQUITY UPLC System
 
 

This work demonstrates the accurate and precise quantification of the endogenous protein, Apolipoprotein A1 in plasma. Using the ProteinWorks eXpress Direct Digest Kit with 3-step protocol, the total sample preparation time was under 1.5 hrs. Through direct digestion of 15 μL of plasma, quantification limits of 5–1500 μg/mL were achieved, while maintaining linearity, precision and accuracy. This method easily distinguishes 5 μg/mL changes in Apo A1 in rat plasma and changes of 75 μg/mL over the endogenous level in human plasma. We’ve demonstrated that using a simple kit-based approach can eliminate the need for method development and allow bioanalytical labs with limited experience to quickly generate robust, accurate and precise data.


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