The development of a fast and simple analytical method for the routine simultaneous identification and quantification of a variety of fatty acids (FA) is desirable for use in various fields. The profiling of fatty acids has mainly been carried out by Gas Chromatography (GC). However, the fatty acids need to be converted to their esters. In addition, the non-volatility of long-chain fatty acid esters and the thermally labile property of unsaturated fatty acids can further complicate GC analysis.
Reversed-Phase Liquid Chromatography (RPLC) has been widely employed for fatty acid determination. RPLC based methods do not require derivatization, and there is no concern about the compound’s volatility, nor their high temperature stability. However, RPLC is less efficient in fatty acid separation than GC is.
UltraPerformance Convergence Chromatography™ (UPC²) is the next-generation supercritical fluid chromatography (SFC) system that provides excellent separation efficiency and speed in a wide range of application areas, including edible oils, acylglycerols, and short-chain fatty acids.
In this technology brief we demonstrate the capability of UPC²-MS for the separation of positional and geometrical fatty acid isomers, and its feasibility for fatty acid profiling.