Characterization of Biotherapeutics: ACQUITY UPLC H-Class Bio with 2D Part 2 of 3: Rendering a Viable Interface for IEX with ESI-MS Analysis

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Robert Birdsall, Sean M. McCarthy, and Weibin Chen
Content Type:
Application Notes
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Application Notes
Related Products:
Xevo G2-S QTof

Characterization methods of biotherapeutic proteins that provide increased informational content without compromising productivity require efficient solutions that are adaptable to the high-throughput environment of industry. The ACQUITY UPLC H-Class Bio System with 2D Technology offers an efficient method for the characterization of biotherapeutics with the ability to combine orthogonal detection techniques on-line for improved productivity.

The heart-cut feature offered with the system is well suited for fractionation and desalting of challenging biological samples. Compatibility with multiple column configurations and the ability to automate the process offers today’s analyst a flexible and efficient means to maximize the information obtained during characterization of biotherapeutics.

In the second part of this three-part series, we address how to successfully interface ion exchange chromatography (IEX) with ESI mass spectrometry as a complementary orthogonal technique to monitor lysine variants.

Part 1: On-line Desalting

Part 3: On-line Enrichment of Low Abundance Species

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Download Part 2 of 3 (720005328en) PDF 2723.36kB