Characterization of Biotherapeutics: ACQUITY UPLC H-Class Bio with 2D Part 1 of 3: On-line Desalting of Biotherapeutic Samples for Increased Productivity

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Robert Birdsall, Sean M. McCarthy, and Weibin Chen
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Application Notes
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Application Notes
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The objective of this three-part application series is to collectively demonstrate that the ACQUITY UPLC H-Class Bio System with 2D Technology offers advantages for the on-line fractionation and desalting (part 1) of challenging biological samples and is a viable interface for IEX with MS analysis (part 2). The system’s ability to increase productivity across platforms will also be demonstrated with the on-line enrichment of low abundance species for peptide analysis (part 3). A therapeutic monoclonal antibody, infliximab, was used to demonstrate this.

Increasing demand for informational content in the characterization of biotherapeutics, combined with a fast-paced work environment, require efficient solutions that offer flexibility. The ACQUITY UPLC H-Class Bio System with 2D Technology is Waters’ solution to these challenges. The heart-cut feature offered with the system is well suited for fractionation and desalting of challenging biological samples. Compatibility with multiple column configurations and the ability to automate the process offers today’s analyst the flexibility needed to maximize information in the characterization of biotherapeutics without compromising productivity.

Part 2: Direct Interface for IEX with ESI-MS

Part 3: On-line Enrichment of Low Abundance Species

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Download Part 1 of 3 (720005329en) PDF 2446.9kB