A Clinical Research Method for the Analysis of Serum Testosterone and Androstenedione

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Dominic Foley and Lisa Calton
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An analytically sensitive and selective clinical research method has been developed for the analysis of serum testosterone and androstenedione.

Excellent method precision was achieved through the use of analytically sensitive and selective sample preparation, chromatography, and MRM mass spectrometry on the ACQUITY UPLC I-Class UPLC System and Xevo TQD. In addition, method accuracy for the analysis of testosterone has been demonstrated through evaluation of CDC HoSt samples.

The sample preparation has been automated using the Tecan Freedom Evo 100/4 Liquid Handler, significantly reducing sample handling time and improving laboratory efficiency with sample tracking capabilities. Both a manual and the automated extraction methods have been shown to be equivalent, providing the user with flexibility in sample preparation options.

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