This technology brief demonstrates the analytically sensitive and precise measurement of adrenal steroids from limited-volume clinical research serum samples.
The data presented indicates the ACQUITY UPLC I-Class (FTN) with Xevo TQD is capable of meeting the analytical precision necessary for measurement of physiologically-relevant concentrations of serum 17-OHP for clinical research. The narrow diameter tubing of the ACQUITY UPLC I-Class System creates a low-dispersion system allowing for greater LC peak capacity and precision than achieved before with the ACQUITY UPLC System . The inclusion of active pre-column heating of mobile phase brings further advantages in terms of column efficiency, improving reproducibility of peak area integration. Precision at low concentrations contributes to overall analytical sensitivity (Table 2). With further optimization of LC methods to improve detection of A4, this configuration can be used in laboratories with low to moderate detector sensitivity needs.