In this application note, we have demonstrated the successful chiral separations of fragrance compounds on ACQUITY UPC2 Trefoil AMY1 and CEL1 Columns using an ACQUITY UPC2 System. The low system volume and extra-column volume of the UPC2, combined with the reduced particle size of the ACQUITY UPC2 Trefoil AMY1 and CEL1 Columns, enable superior, faster, and more efficient separations compared with traditional SFC and GC. The demonstrated analysis times range from 2 to 3 minutes, significantly shorter than the 15-50 minute analysis time typical for chiral GC,3 allows for a fast authentication of the natural sources of essential oils. In all cases, the closely eluting isomers were baseline resolved. For the essential oil analysis, the oil samples were diluted and directly injected onto an ACQUITY UPC2 System without tedious sample preparation. Furthermore, the inherent compatibility between UPC2 and MS offered an unambiguous identification of the target analytes in a complex sample matrix. The high efficiency, short analysis time, and simple sample workup demonstrated in this study should be welcomed by industries where chiral analyses of fragrance compounds are routinely performed.