The analysis of flavonoids in citrus species is often used for quality control and product authenticity because of their remarkable taste properties and variation of flavonoid profiles in the different species and varieties of citrus. The ACQUITY UPLC H-Class System with the ACQUITY QDa Detector offers the best balance of selectivity, usability, and affordability for flavonoids analysis. The ACQUITY QDa Detector is designed to provide mass spectral data with minimal tuning at an affordable cost. It not only extends the sample detection coverage of optical detection to compounds with no UV or fluorescence chromophore, but it also improves the selectivity of compounds of similar structures, which is very useful in flavonoids analysis.
This application note describes a fast, simple, and reliable method for the QC and profiling of flavonoids in citrus species using the ACQUITY UPLC H-Class System and the ACQUITY QDa Detector.