Multiplexed Analysis of Steroid Hormones Using ionKey/MS

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Carolyn Broccardo, James P. Murphy, Giuseppe Astarita, and Jessica Prenni
Waters, Colorado State University
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In this application, we report the use of the newly developed 150-μm ionKey/MS System for the multiplexed quantitation of five steroid compounds in human serum. The use of the ionKey Source and the 150-μm iKey Separation Device yielded a 100-400 fold increase in on-column sensitivity while at the same time decreasing solvent usage by 150 fold as compared to standard flow methods. The increased on-column sensitivity allowed for simplification of the steroid extraction procedure which in turn streamlined the sample preparation and reduced per/sample assay cost.

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