Simultaneous in vivo Quantification and Metabolite Identification of Plasma Samples Using High Resolution QTof and Routine MSE Data Analysis

Library Number:
APNT134742818
Part Number:
720004723EN
Author(s):
Mark Wrona, Paul Rainville, Eric Langlois, Nigel Ewing, and Julie Laterreur
Source:
Waters, Vertex Biopharmaceuticals
Content Type:
Application Notes
Content Subtype:
Application Notes
Related Products:
 
 
 
Xevo G2 QTof
 
 
 

This application note summarizes the collaboration between Vertex and Waters to test and demonstrate a quan/qual workflow to determine robustness and reliability of QTof measurements, and compare them against the gold-standard tandem quadrupole workflows.

In this example, propranolol was used as a model compound and a pharmacokinetic (PK) rat study (containing standard curves, PK runs, and QCs) was conducted by Vertex Pharmaceuticals using a Xevo G2 QTof MS System for analysis. The sample set was also run at Vertex using a triple quadrupole platform with a typical MRM-based workflow under the same chromatographic conditions to provide a reference comparison data set.


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