Dyes are added to change or add color to a product, with the aim to add appeal and improve sales by making the product more authentically pleasing. Dyes are used in many products, for example industrial products such adhesive glues and industrial cleaning products; agricultural products such as seed colorants; cosmetics products (for example lipstick and eye shadow); personal care products (for example soaps, hair dye, and wigs); consumer products (for example inks, candles, fabric, paper, and leather); automotive products (for example car washes and polishes).
The standard method for the analysis of disperse dyes in textile products and components is DIN54231, using high performance liquid chromatography (HPLC) or thin layer chromatography (TLC) with either ultraviolet (UV), mass spectrometry (MS), or densitometry detection. Other methodologies for the analysis of disperse dyes include: electrochromatography with electrospray ionization (ESI) and MS detection, HPLC with: UV/VIS detection, atmospheric pressure chemical ionization (APCI) and MS detection, ESI and MS detection, and ion-exchange high-performance liquid chromatography (HPIEC) with MS detection.
In this application note, we describe the advantages of analyzing disperse, acid, direct, and basic dyes using the ACQUITY UPLC H-Class System coupled with the Xevo TQD. Our results show increased robustness, selectivity, and sensitivity, with reduced run times and associated savings in solvent usage compared to existing methodologies.