Optimization of Trypsin Digestion for MRM Quantification of Therapeutic Proteins in Serum

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A general workflow for the quantification of therapeutic proteins in serum without analyte pre-fractionation was tested to determine the quantification of trastuzumab in human serum. The analytical workflow of trypsin digestion can be optimized to develop a successful multiple reaction monitoring (MRM) assay. Results include higher assay sensitivity, reduced sample preparation time, and higher assay reproducibility.

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