The presence of flavonoids in citrus juices has attracted attention because of their biological and physiological benefits. Considering these associated health benefits, the impact of food processing and preparation on flavonoid content demands a greater understanding. It is, therefore, essential to be able to profile all major and minor components of fruit juice to enable a better understanding of dietary consequences. Also, the impact of important factors that can influence consumers, such as appearance and taste, influence the commercial value of a product. In this application note, the use of the ACQUITY UPLC system combined with a SYNAPT G2-S Mass Spectrometer, to provide specific and unambiguous identification of the components present in citrus juices. High Definition Mass Spectrometry (HDMS) has been utilized to profile citrus juice products, and this technique offers some unique advantages for profiling complex mixtures. It is a combination of high resolution mass spectrometry and high efficiency ion mobility based measurements and separations. Ion mobility spectrometry (IMS) is a rapid, orthogonal gas separation phase technique that provides another dimension of separation within an LC time frame, offering higher ion definition and analytical specificity. Compounds can be differentiated based on size, shape, and charge, as well as mass.