Two-Dimensional Liquid Chromatography for Quantification and MS Analysis of Monoclonal Antibodies in Complex Samples

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Sean M. McCarthy, Thomas E. Wheat, Ying Qing Yu, Jeffrey R. Mazzeo
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Using the ACQUITY UPLC H-Class Bio System with 2D technology, a method was developed to determine monoclonal antibody concentration and mass profile from a single injection. The system delivered the necessary reproducibility in terms of retention time, recovery, and response for samples from complex matrices using both UV and MS detection.

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