Recently, the use of mass spectrometry (MS) for the detection of allergens has generated much interest due to its ability to target the specific protein and to analyze multiple allergenic markers in a single method. In this technology brief, an approach for the detection of peptide markers of allergens in a food matrix is demonstrated using peanut in bread. An important advance in instrument design that enables the simultaneous acquisition of matrix background information during the quantitative MRM analysis is also illustrated. This functionality (known as RADAR acquisition) offers an innovative approach for the quantification of analytes of interest while monitoring background interferences. This enables informative decisions to be made during method development and continuous monitoring of the matrix background to ensure a robust and reliable method.