Characterizing High-Order Structures of Therapeutic Proteins by Electrospray Ion-Mobility MS

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Weibin Chen, Asish B. Chakraborty, StJohn Skilton, Scott Berger and Jeff Mazzeo
Waters Corporation
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This poster, P-106, was shown at Mass Spec 2010 (Practical Applications of Mass Spectrometry in the Biotechnology Industry), Marina Del Ray, CA, Sept. 8-10, 2010.


  • Reproducible safety and efficacy profiles for biotherapeutic proteins require manufacturers to produce products with consistent tertiary structures
  • Methods for determining changes in higher order structure are invaluable for the assessment of product quality
  • In this work, Waters SYNAPT® G2 HDMS™ is used to characterize biotherapeutic protein tertiary structures and data from orthogonal analytical techniques is applied to corroborate the IMS results
  • Cross-sectional areas measured by IMS method are in good agreement with those calculated using the MOBCAL computations



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