Analytical HPLC to Preparative HPLC: Scale-Up Techniques using a Natural Product Extract

Library Number:
APNT10117943
Part Number:
720003120en
Author(s):
Andrew Aubin and Ronan Cleary
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
Dimensions:
SunFire C18 5 um 4.6x50 mm column (186002557) SunFire C18 5 um 10x50 mm column (186002561) SunFire C18 5 um OBD 19x50 mm column (186002566) SunFire C18 5 um OBD 30x50 mm column (186002570)
Related Products:
 
3100 Mass Detector
 
3100 Mass Detector
 
 
 
 
 
 
 

Using the Waters AutoPurification System, separation methods can be developed on an analytical scale and transferred to preparatory scale on the same system, reducing a laboratory's overall capital investment. Here we illustrate a systematic approach to scale-up using the separation of Kudzu (Pueraria lobata) root extract to move from a 4.6 mm I.D. analytical column separation through 10, 19, and 30 mm I.D. preparatory column separations. The Waters OBD Prep Calculator is featured.


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