Effective Use of Temperature Control in Compound Isolation

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Jo-Ann M. Jablonski, Thomas E. Wheat, Diane M. Diehl
Waters Corporation
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Application Notes
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Technical Notes
10101326 XBridge Prep OBD C18 5 µm 19 x 150 mm
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All chemical processes are temperature sensitive.  As the temperature rises, viscosity and pressure decrease, retention decreases, and the selectivity of the separation changes.  While temperature can effectively improve a separation, it is seldom used at the preparative scale.  Lack of effective temperature control results in preparative chromatograms that are not comparable to the analytical separation, making compound isolation more difficult.  This work illustrates an effective solution for controlling temperature of large columns at high flow rates.

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