Knowledge of the efficacy of the individual enantiomers of pesticides is of great interest to agrochemical companies. The benefits of using these products can be realized only when the optimal application rates are determined and when the appropriate metabolic and toxicological evaluations are performed. However, the ability to separate the enantiomers is required in order to study the effects of each individual isomer.
Experiments concerning the steroeselective behavior of the individual pure isomers of chiral pesticides depend on the development of the necessary preparative separation technology that enables them to be readily separated and isolated as single stereoisomers.
Although traditionally dominated by liquid chromatography (LC), there is an increasing trend toward using supercritical fluid chromatography (SFC) to replace normal phase LC for chiral purifications from the semi-preparative scale up to the kilogram scale.
In this application note, we present the chiral resolution of metalaxyl using the ACQUITY UPC2 System, followed by scaling to a semi-preparative purification using the Investigator SFC System. this workflow is ideal for agrochemical companies facing escalating pressure to discover, develop, and release new products into the marketplace while adhering to strict deadlines and budget constraints.