A Validated Liquid-Liquid Extraction Method with Direct Injection of Hexane for Clopidogrel in Human Plasma Using UltraPerformance Convergence Chromatography (UPC2) and Xevo TQ-S

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Jennifer L. Simeone and Paul D. Rainville
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Application Notes
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Application Notes
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Ultra High Sensitivity Bioanalysis

This application note demonstrates the use of convergence chromatography in the development and validation of a highly sensitive UPC2/MS/MS assay for the direct analysis of clopidogrel in human plasma from a hexane LLE preparation. The assay showed excellent intra- and inter-day accuracy and precision in a three-day validation study. The level of quantification was determined to be 25.0 pg/mL with a signal-to-noise ratio of approximately 60:1 with the %CV and bias both less than +/- 15%. The carryover was determined to be significantly less than 20% of the LLOQ in an extracted blank, following the injection of a high concentration standard.

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