Separation and Characterization of N-linked Glycopeptides on Hemagglutinins in a Recombinant Influenza Vaccine

Library Number:
APNT10123493
Part Number:
720003173en
Author(s):
Hongwei Xie, Weibin Chen, Martin Gilar, St John Skilton, and Jeffery R. Mazzeo
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
Related Products:
 
 
ACQUITY UPLC System
 
 
 
ACQUITY UPLC System
 
 

In this application note, we demonstrate that UPLC/MSE is capable of separating and characterizing N-linked glycosylations of hemagglutinin (HA) proteins in a recombinant influenza vaccine candidate expressed from insect cell-baculovirus expression vector system (BEVS). Glycopeptides and glycoforms are separated by an ACQUITY UPLC System at the peptide level, and are detected online by a SYNAPT MS System. The UPLC/MSE data are processed by BiopharmaLynx Software to report N-linked glycosylation information. The method offers a way to improve the characterization as well as reduce the amount of time spent on data processing. Furthermore, having a general technique that can be applied to such problems opens this type of analysis to non-experts and could benefit an organization by streamlining their work.


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