HPLC Essentials

  • Overview
  • Venue
Date(s): January 15, 2020
Time: 8:30am - 3:00pm
Location: Columbia, MD
Language: English


In this informational seminar we will discuss the fundamentals of HPLC. Understanding the importance of the basics will enable you to be more effective in your laboratory. We will discuss the different types of modern LC instrumentation and how to efficiently utilize the instrumentation. We will spend time discussing how to troubleshoot your system and share best practices to ensure you are getting maximum efficiency out of your system and methods.


  • HPLC instrument options
  • System Care and Use
  • Troubleshooting Tips and Tricks
  • Benchmarking and Vials


  • Are you aware of the differences between types of HPLC instrumentation?
  • Have you used other analytical technologies but are new to liquid chromatography?
  • Do you have instrumentation in the lab that could run more efficiently?
  • Would you like to learn some troubleshooting tips and tricks?


  • Lab analysts new to chromatography
  • Experience analysts looking for a refresher on liquid chromatography
  • Anyone who would like to learn more about HPLC


8:30am  Arrival and Registration 
9:00am  Welcome and Opening Remarks
9:15am  HPLC System Options
This presentation will cover the different configurations of HPLCs available and how to best determine what is right for your analysis.  
10:00am  Break
10:15am  Chromatography Systems Best Practices
This presentation will cover mobile phase considerations, passivation, system storage and cleaning
11:00am Method Considerations
This presentation will look at dispersion and dwell volumes of various LC instruments and tips and tricks when switching systems or vendors.
11:45am  Lunch
1:00pm  Maximizing System Efficiency: The Importance of Benchmarking and Vials
This presentation covers two topics that can help analysts ensure the quality of their data and avoid problems: The use of standards to benchmark and monitor system health, and the importance of the choice of vials.
1:45pm  Troubleshooting Tips and Tricks
This presentation will look at some common LC issues and how to correct them.
2:30pm  Troubleshooting Contamination and Carryover
This presentation will go over suggested troubleshooting for contamination and carryover.
3:00pm  Summary and Closing Remarks

 For more information or questions, please contact Mike_Eicher@waters.com