Due to the polarity and nature of the target analytes, retaining and separating polar species pose specific and challenging problems for the chromatographer. The optimized particle pore size and C18 surface concentration of the T3 sorbent chemistry enables reliable operation with 100% aqueous mobile phases needed for retaining highly polar analytes. Waters offers a range of CORTECS Solid-Core T3, XSelect HSS T3, ACQUITY UPLC HSS T3, and Atlantis Silica T3 Columns and method validation kits based on your separation requirements.
Many separation scientists prefer reversed-phased chromatographic approaches to retain polar compounds. T3 particle technology is derived from C18 ligands and proprietary end-capping to achieve optimum characteristics suited for polar compound retention. Three stationary phase characteristics define the T3 columns for polar retention:
Optimized pore size - Pore size plays a critical role to dramatically reduce retention loss due to stationary phase dewetting (hydrophobic collapse) when using highly aqueous mobile phases. Compatibility with 100% aqueous mobile phases is critical to maximize reversed-phase retention for highly polar species.
Bonded alkyl functionality - At low pH (<2) the rate of acid-catalyzed cleavage of the bonded ligands increases significantly. The result of this hydrolysis is a decrease in retention over time and pronounced peak tailing. Consequently, column lifetime is dramatically reduced. Proprietary trifunctional T3 bonding and end-capping technology yields packing materials with superior low pH stability.
Optimized surface coverage and ligand density - Using controlled manufacturing processes using specifically treated silica base particles results in a uniquely bonded and encapped sorbent that reproducibly maximizes polar compound retention. The T3 bonding process is shared across the T3 Families to ensure column-to-column and batch-to-batch reproducibility.
ACQUITY UPLC and XSelect HSS T3 Columns
The universal, High Strength Silica (HSS)-based bonded phase used for the ACQUITY UPLC HSS T3 (1.8 µm particle size) and XSelect HSS T3 (2.5-5 µm particle size) sorbents is compatible with 100% aqueous mobile phase and should be your first choice when developing reversed-phase separations for polar compounds. Fully scalable from UPLC (1.8 µm particle)-HPLC (2.5-5 µm particle)-preparative OBD (5 µm particle) column formats, the HSS T3 packing is the most universal reversed-phase sorbent for polar compound retention. All HSS T3 columns exhibit ultra-low MS bleed, long column lifetime at low pH, and mechanically stable packed beds.
ACQUITY UPLC and XSelect HSS T3 Columns are designed for reproducible performance that allows:
CORTECS Solid-Core T3 Columns
CORTECS T3 Columns provide conventional C18 behavior for polar and non-polar species when using mobile phases with low percentages of organic modifier (up to 100% aqueous conditions). The result is easier and faster method development for a wide range of polar and non-polar compounds using a single CORTECS T3 Column.
CORTECS T3 Columns ensure:
Atlantis T3 Columns
Atlantis T3 HPLC columns contain fully-porous silica-based, reversed-phase C18 sorbents that display high retention and loading for highly polar compounds. The ability to inject high mass loads of polar compounds corresponds to less preparative runs, faster library screening and purification.
The intermediate ligand density of the Atlantis T3 sorbent provides an optimal balance between ligand coverage and a low concentration of residual surface silanols. Traditional endcapping, for example, is not sufficient to reduce undesirable silanol activity with polar alkaline species. The proprietary T3 endcapping process allows for higher surface coverage compared to trimethyl silane (TMS) endcapping, which results in higher analyte retention while maintaining superior peak shape.
Atlantis T3 Columns are your first choice when you require: