Mixed Mode for Released Glycans

Supreme Resolution of Charged Glycan Species

The ACQUITY PREMIER Glycan BEH C18 AX Columns are anion-exchange RP columns that provide charge-based separations and extra resolution for acidic glycans. With the mixed-mode separation, glycans are grouped by different charged states, and retention is increased to analyte net charge. Additional resolution of isomeric glycan species within the same charged group can be achieved due to the RP properties of the stationary phase.

MaxPeak High Performance Surfaces

It is often a challenge to analyze acidic glycans due to their propensity to be lost to columns' metallic surfaces. The ACQUITY PREMIER Glycan BEH C18 AX Column hardware is outfitted with specialized inert surfaces, MaxPeak High Performance Surfaces. This introduces a barrier layer preventing acidic glycans from undergoing undesired secondary interactions inside the column. In turn, glycan recoveries are improved, and requirements for sample conditioning upon first time use is eliminated. 

MS and Multiple Label Compatibility

Unlike other commercially available mixed-mode stationary phases, the ACQUITY PREMIER Glycan BEH C18 AX stationary phase is based on a highly stable ionizable modifier and bonding procedure. This makes it ideally suited for separating glycans derivatized by MS-enhancing labels with amphiphilic, strongly basic moieties, such as Waters RapiFluor-MS. It is also proven to be useful for 2-AB derivatized glycans.

Charge-Based Glycan Solution

The ACQUITY PREMIER Glycan BEH C18 AX Columns are integrated with an easy-to-use, MS-grade mobile-phase concentrate and the RapiFluor-MS Sialylated Glycan Performance Test Standard, providing a complete charge-based separation solution. 

Optimized Sample Preparation

The GlycoWorks RapiFluor-MS N-Glycan Kit reduces complicated, time-consuming sample preparation.

• Streamline sample preparation
• Integrate automation
• Reducing protocols for disulfide-rich proteins