Determination and control of critical nutrients in cell culture is important to maximize protein yield and provide a consistent manufacturing process. The Waters UPLC®Amino Acid Analysis (AAA) Solution is a turnkey solution that provides accurate, high throughput amino acid analysis.
The UPLC Amino Acid Analysis Solution meets the specific demands of analyzing amino acids for protein characterization, cell culture monitoring, and nutritional analysis of foods and feeds. This end-to-end system is designed to deliver the accuracy and reliability you need to be successful.
UPLC Amino Acid Analysis Solution
The UPLC Amino Acid Analysis Solution provides you with control over the major factors that commonly cause variability in amino acid analysis. From optimized instrumentation and quality controlled chemistries to standardized software projects, methods, report templates, and qualification tools, you will rely on a method that is both rugged and reliable – a comprehensive and detailed approach that ensures successful analysis of every sample.
The UPLC AAA System brings together industry-leading technologies—instrumentation, chemistries, software, qualification tools and services – that are specifically optimized for exceptional, application-focused performance. Designed by scientists for scientists, these solutions remove the guesswork in choosing the best solution for your application’s needs.
Importance of Amino Acids
Analysis of amino acids is required in several areas of research, and it is a fundamental tool in product analysis. The application imposes different requirements on the analytical method because the amino acids play different roles.
With the UPLC Amino Acid Analysis Solution, you benefit from better resolution and sensitivity, all achieved in a shorter analysis time than with conventional methodologies:
Optimized Peak Identification
AccQ•Tag™ Ultra Chemistries used in the UPLC Amino Acid Analysis Solution are a comprehensive and fully-tested set of reagents, columns, and eluents optimized for use with the ACQUITY UPLC H-Class and ACQUITY UPLC H-Class Bio Systems. With superior resolution of all amino acids, you will confidently achieve peak identification and quantitation, which in turn assures the accuracy of your results.
This chemistry is based on Waters’ widely-used and understood AccQ•Tag derivatizaton method. Primary and secondary amino acids react with a single reagent, AccQ•Fluor™, in a high-throughput batch process resulting in exceptionally stable derivatives. High resolution separations are achieved using pre-qualified AccQ•Tag Ultra UPLC columns and mobile phases. Derivatized amino acids are quantified to sub-picomole levels with single wavelength UV detection.
Performance Qualification
Qualification services available for the UPLC Amino Acid Analysis Solution meet the most stringent regulatory requirements. Installation, Operation, and Performance Qualification (IQ/OQ/PQ) protocols ensure that every element of the system is performing according to its intended use. An additional PQ, specific for amino acid analysis, is also available to verify performance and ensure accurate results.
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ResolutionAnalysis of a Hydrolysate Standard at 10 pmol/mL The UPLC Amino Acid Analysis Solution provides high resolution to ensure method robustness with good quantitation. The short cycle time is obtained without compromising accurate results. |
EfficiencyEfficient, Reliable Foods and Feed Sample Analysis: Soybean Meal Hydrolysate Two samples of hydrolyzed soybean meal were analyzed. The upper trace shows the untreated sample while the lower trace shows the results of performic acid oxidation. Note the appearance of cysteic acid and methionine sulfone in the oxidized sample. |
Determination and control of critical nutrients in cell culture is important to maximize protein yield and provide a consistent manufacturing process. The Waters UPLC®Amino Acid Analysis (AAA) Solution is a turnkey solution that provides accurate, high throughput amino acid analysis.
The UPLC Amino Acid Analysis Solution meets the specific demands of analyzing amino acids for protein characterization, cell culture monitoring, and nutritional analysis of foods and feeds. This end-to-end system is designed to deliver the accuracy and reliability you need to be successful.
Flexible MethodologyAnalysis of Hydrolysate and Cell Culture Standards The upper trace shows the analysis of a hydrolysate standard at 10 pmol/mL, and the lower trace a standard mixture of the larger number of amino acids found in cell culture media, also at 10 pmol/mL. The adaptation of the method requires only a change in dilution of the Eluent A Concentrate supplied as part of the total system solution. |
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Cell Culture AnalysisDirect Cell Culture Sample Analysis Aliquots of cell culture media were taken directly from a bioreactor producing a biopharmaceutical protein. Samples were taken over a period of six days and no special sample preparation was required before the amino acid analysis shown here. The amino acid concentration trends can be plotted with growth as well as the response to feeding. |