Summary and References



With the practices described, robust ACQUITY UPLC Protein BEH SEC analyses can be achieved, offering faster and higher resolution separations than those obtained using traditional HPLC-based SEC methods. To maximize the lifetime of an ACQUITY UPLC Protein BEH SEC Column, it is important to avoid microbial growth in the mobile phase as well as to ensure that injected samples are free of particulates. The use of BEH SEC guard columns can be an effective means to protecting your analytical SEC column from deterioration due to these various types of failure mechanisms.



1. Hong, P.; Koza, S.M.; Bouvier, E.S.P. A Review: Size-exclusion chromatography for the analysis of protein biotherapeutics and their aggregates. J. Liq. Chrom. Rel. Tech. 2012, 35(20), 2923-2950.  

2.Bouvier, E.S.P.; Koza, S.M. Advances in size-exclusion separations of proteins and polymers by UHPLC. Trends Analyt. Chem2014, 63, 85-94.

3. Guideline for Disinfection and Sterilization in Healthcare Facilities, 2008, William A. Rutala, David J. Weber, M.D., and the Healthcare Infection Control Practices Advisory Committee. US CDC, Atlanta, Georgia. (

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