The innovative iKnife hand-held sampling device produces information-rich vapor directly from the surface of the sample, which when analyzed by a time of flight (Tof) MS, provides analysts with an accurate molecular profile in seconds.
*The REIMS Research System with iKnife Sampling is intended for research use only and is not intended for diagnostic or therapeutic use
No sample preparation or chromatography required
MS-based analysis of complex sample matrices often requires sample preparation and chromatographic separation prior to the MS detection. This approach can be time consuming and costly, particularly when large numbers of samples or replicates are required. It can also be technically challenging and can introduce unwanted selectivity to the analysis.
The REIMS Research System with iKnife Sampling removes the need for sample preparation and chromatographic separation, allowing an accurate mass MS profile to be collected in the space of a just few seconds per sample. This saves time and money, and makes it easier than ever before to obtain detailed molecular information from a range of sample types.
Furthermore, the long, flexible connection between the iKnife handheld sampling device and the MS allows greater freedom of movement than any other ionization technique, bringing the analysis to the sample.
Quickly determine differences within and between samples
With molecular profile data collected from multiple samples of similar type or origin, it is often not possible to determine significant differences by visual inspection, especially since the differences are often represented by changing ratios of multiple components rather than the presence or absence of a few key species.
Progenesis QI software offers an intuitive, workflow-based approach to process large data sets using powerful multivariate statistical methods. This quickly highlights similarities and differences between samples, allowing them to be grouped in an objective and unbiased way.
Identify molecular markers responsible for sample differences
Once samples have been grouped according to their similarities and differences, it is important to understand the reasons for those differences in order to provide a more detailed understanding of the chemical or biological system under investigation.
Progenesis QI highlights spectral features that cause the differentiation between sample groups, and then uses the accurate mass MS data to search databases and identify the molecular markers. These identifications can then be confirmed for a greater level of confidence, for example by conducting targeted MS/MS experiments to obtain additional structural information.
Available on IMS MS/MS or MS/MS
Waters QTof systems provide the ideal platform for REIMS with iKnife sampling, with superior performance, proven robustness and reliability, and the flexibility to adapt to changing priorities and future needs.
SYNAPT G2-Si HDMS
For the ultimate research capabilities
For accessible, routine sample analysis
Potential to combine with UPLC/MS towards a holistic profiling approach
Both SYNAPT G2-Si HDMS and Xevo G2-XS QTof are compatible with a wide range of Waters chromatography inlets and ionization sources. This allows aliquots of the same sample to be analyzed on a single mass spectrometry system using multiple different analytical techniques, for example REIMS, UPLC-MS and GC-MS, towards a holistic profiling approach.
UPLC, Nanoflow UPLC, UPC2, GC, APC
ESI, nanoESI, ionKey/MS, APGC, APCI, ESCI, ASAP, DESI, MALDI*
*MALDI available on SYNAPT G2-Si HDMS only