Atlantis and Atlantis PREMIER Columns

Solving One of the Most Challenging Chromatographic Problems, Retaining Polar Compounds

Atlantis Columns are designed to retain and separate polar analytes; and provide quick, accurate, and reproducible quantification with increased retention and sensitivity.

This column family provides exceptional performance, versatility, retention, and chromatographic stability for polar compounds, while also affording balanced retention for broad analyte mixtures. With the legacy T3, dC18, and HILIC chemistries available in the standard Atlantis Column configurations, additional Atlantis chemistries are available in the PREMIER Class configuration.

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Selectivity comparison: polar acid, base, neutral mixture

Atlantis PREMIER BEH C18 AX Columns

Atlantis PREMIER BEH C18 AX is the next evolution of columns in the Atlantis family. These reversed-phase/anion-exchanged-mixed-mode columns utilize hybrid particle technology, and are designed to provide exceptional results in a variety of reversed-phase polar analyte applications.

Atlantis PREMIER BEH C18 AX Columns build on Waters industry-leading polar retention chemistries.

Built using a high retention BEH 95 Å particle
Utilize MaxPeak High Performance Surfaces (HPS) to reduce analyte/surface interactions
Employ a charge modifier that provides novel selectivity and increased retention for acids over a wider pH range
Available in both a column and VanGuard FIT column format (an easy-to-use integrated guard design that extends column life)

Atlantis T3 Columns, Ideal for Diverse Analyte Mixtures

Atlantis T3 Columns are a universal, silica-based, reversed-phase C₁₈ line of HPLC columns that not only retain and separate small, water-soluble polar organic compounds, but also provide superior performance across a wider pH range.

For reversed-phase applications, Atlantis T3 Columns should be considered the first choice when developing a separation of polar (and non-polar) compounds. The advantages of T3 bonding technology is extended to UPLC in the ACQUITY UPLC HSS T3 Columns.

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Superior retention and peak shape under 100% aqueous conditions

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Retention Mechanisms in Hydrophilic Interaction Chromatography

Polar Compounds Retention without Ion-Pairing Reagents

Eliminating ion-pairing reagents improves detection limits, method reproducibility and robustness, while reducing instrument maintenance due to harsh mobile-phase environments.

Atlantis HILIC Silica Columns are designed to retain very polar, organic molecules that are too polar to retain by RP. Unlike the highly aqueous mobile phases required for polar retention in RP separations, Atlantis HILIC Silica Columns employ highly volatile (>80% organic) mobile phases which are ideal for mass spectrometry (MS) response and sensitivity. Additionally, direct compatibility with high organic SPE eluates dramatically increases the number of samples that can be handled, thus substantially increasing sample throughput.

Preparative Chromatography

Atlantis T3 and HILIC Silica Prep OBD (Optimum Bed Density) Columns offer analytical column performance in preparative chromatography formats to deliver a variety of advantages including:

Improved bed stability
Higher efficiency
Longer column lifetime
Increased loadability

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Comparison of Atlantis T3 and Conventional C18 Polar Compound Preparative Separation

Atlantis T3 Prep OBD Columns enable the successful isolation and purification of polar species. Atlantis HILC Silica Prep OBD Columns employ high organic mobile phases that lead to lower viscosity and lower backpressure separations with faster evaporation of collected fractions.