Atlantis Columns

Atlantis T3 Columns, Ideal for Diverse Analyte Mixtures

Atlantis T3 Columns are a universal, silica-based, reversed-phase C₁₈ line of HPLC columns that not only retain and separate small, water-soluble polar organic compounds, but also provide superior performance across a wider pH range.

For reversed-phase applications, Atlantis T3 columns should be considered the first choice when developing a separation of polar (and non-polar) compounds. The advantages of T3 bonding technology is extended to UPLC in the ACQUITY UPLC HSS T3 columns.

Polar Compounds Retention without Ion-Pairing Reagents

Eliminating ion-pairing reagents improves detection limits, method reproducibility and robustness, while reducing instrument maintenance due to harsh mobile-phase environments.

Atlantis HILIC Silica Columns are designed to retain very polar, organic molecules that are too polar to retain by RP. Unlike the highly aqueous mobile phases required for polar retention in RP separations, Atlantis HILIC Silica Columns employ highly volatile (>80% organic) mobile phases which are ideal for mass spectrometry (MS) response and sensitivity. Additionally, direct compatibility with high organic SPE eluates dramatically increases the number of samples that can be handled, thus substantially increasing sample throughput.

VanGuard Column Protection

The often complex sample matrices encountered in pharmaceutical, natural product, environmental, and industrial chemical analysis prematurely shorten analytical column lifetime and degrade chromatographic performance. Waters VanGuard Cartridge Columns help improve analytical column performance by removing particulate and chemical contamination that may be present in the mobile-phase stream—without compromising chromatographic performance.

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Preparative Chromatography

Atlantis T3 and HILIC Silica Prep OBD (Optimum Bed Density) Columns offer analytical column performance in preparative chromatography formats todeliver a variety of advantages including:

• Improved bed stability
• Higher efficiency
• Longer column lifetime
• Increased loadability

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Comparison of Atlantis T3 and Conventional C18 Polar Compound Preparative Separation

Atlantis T3 Prep OBD Columns enable the successful isolation and purification of polar species. Atlantis HILC Silica Prep OBD Columns employ high organic mobile phases that lead to lower viscosity and lower backpressure separations with faster evaporation of collected fractions.