Where Did My Sample Go? Peptides and proteins are especially prone to non-specific binding (NSB) or adsorption to solid surfaces. These surfaces could be pipette tips, sample storage containers, or injection needles. Let’s examine the consequence of non-specific binding on sample analysis, including low recovery, high variability, poor sensitivity and insufficient linear dynamic range.
What Do Great Vacations and Peptide Bioanalysis Have in Common? Often bioanalysts evaluate the sensitivity, accuracy, and precision of data from a high performance triple quadrupole mass spectrometer as a marker of assay performance, but how often is the influence of good sample handling, preparation, and separation overlooked?