ACQUITY UPLC System

UPLC transforms the expectations of laboratories worldwide

UPLC Technology, which has been adopted successfully in laboratories around the world for the most demanding separations, is highly robust, dependable, and reproducible.

Work at higher efficiencies with a much wider range of linear velocities, flow rates, and backpressures
Higher flow rates and increased throughput result in superior resolution and sensitivity
Eliminate significant time and cost per sample, while improving quality of results
Benefit from Waters’ patented sub-2-μm hybrid particle chemistry

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The ACQUITY UPLC System

The ACQUITY UPLC System eliminates significant time and cost per sample from your analytical process while improving the quality of your results. By outperforming traditional or optimized HPLC, this technology allows chromatographers to work at higher efficiencies with a much wider range of linear velocities, flow rates, and backpressures.

UPLC Technology, which has been adopted successfully in laboratories around the world for the most demanding separations, is highly robust, dependable, and reproducible. What differentiates the holistic design of ACQUITY UPLC systems is Waters’ patented sub-2-μm hybrid particle chemistry, which offers significant benefits over today's HPLC systems equipped with standard 5-μm particle chemistries.

The ACQUITY UPLC System, used on its own or paired with Waters optical and MS detection technologies, provides unique end-to-end solutions for all industries and applications, including: ADME screening, food safety, bioanalysis, clinical, metabolite identification, metabonomics, method development, opens access, and routine quality screens.

Since its invention, ACQUITY UPLC Technology has enabled analytical chemists to reach far beyond conventional HPLC separations.

Read on to discover other ACQUITY UPLC systems optimized for your specific applications, or skip down to see the features of the ACQUITY UPLC System.

ACQUITY UPLC H-Class System

For performing routine analyses or developing methods.

Whether you are performing routine analyses or developing methods, or just prefer the flexibility of multi-solvent capabilities in a quaternary-based system, the ACQUITY UPLC H-Class System is a streamlined system that brings together the flexibility and simplicity of quaternary solvent blending and a flow-through-needle injector to deliver the advanced performance expected of UPLC type separations – high resolution, sensitivity, and improved throughput – while maintaining the robustness and reliability that ACQUITY UPLC systems are known for.

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A generic scouting gradient from 5% to 95% methanol over 15 minutes was performed to investigate the eultion and separation between the peaks.

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ACQUITY UPLC I-Class System

Solve your most complex challenges.

The ACQUITY UPLC I-Class System provides the most powerful solution to the most critical need in separation science today – successfully analyzing compounds that are limited in amount or availability amid a complex matrix, more rapidly than ever before. Developed to produce the most accurate and reproducible separations, you will get the most information possible and accelerate laboratory results.

ACQUITY UPLC M-Class System

For nano- to microscale separations.

The ACQUITY UPLC M-Class System is the system of choice for the highest quality nano- to microscale UPLC separations, for every laboratory performing sample-limited LC/MS analyses. With unprecedented usability in a nano- to microscale platform, the ACQUITY UPLC M-Class System enables laboratories to succeed in analyzing compounds that are severely limited in amount or availability, even amid a complex matrix over several orders of magnitude.

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The ACQUITY UPLC M-Class System and SYNAPT G2-Si delivers high peak capacity separations with exceptional LC/MS data quality.

Features of the ACQUITY UPLC System

Comprehensive system components, including the ACQUITY UPLC Sample Organizer, Column Manager and Heater/Cooler, Binary Solvent Manager and Sample Manager, for customized throughput and condition requirements
Easy HPLC to UPLC method conversion with the ACQUITY UPLC console calculator
Support for existing HPLC methods
Predictive system support with Connections INSIGHT^® remote intelligent services
Chemistries and formats to suit specific analytical tasks, including ACQUITY UPLC 1.7 μm columns, VanGuard pre-columns or ACQUITY UPLC HSS and HSS T3 1.8 μm columns
Extended detection capabilities with ACQUITY UPLC Fluorescence (FLR), Tunable UV (TUV), Photodiode Array (PDA), Evaporative Light Scattering (ELS), and Refractive Index (RI) detectors to single, tandem, and time-of-flight mass spectrometers
Easily compatible with third-party MS solutions (ABI/MDS SCIEX, Bruker Daltonics, and Thermo Fisher Scientific)

ACQUITY UPLC System Advantages

Efficiency gains with sub-2 μm particles:

Provides 3X higher efficiency than 5 μm particles
70% higher resolution than 5 μm particles
For gradient separations, peak capacity is used to measure the resolving power

Better information gained when analyzing more samples per unit time:

Column length can be reduced by a factor of three with methods transitioned from 5 μm particles to sub-2 μm particles, with the same efficiency
Separations can be run at a 3X higher flow rate
Generate a 9X increase in throughput with no loss in resolution

Detect compounds at increasingly lower levels without inherent changes to the detection mode, either optical or MS:

The ACQUITY UPLC System with sub-2 μm particles provides up to 70% higher sensitivity
In the case of equal efficiency but faster analysis times, the equivalent is 3X higher sensitivity than 5 μm particles
Beyond optical detection and looking at MS, the less dispersed peaks with higher concentration effectively yield improved ionization efficiency, making ACQUITY UPLC the ideal inlet for any MS detector

How do chromatographic technologies compare?

Increased Resolution

Waters' cutting-edge UltraPerformance LC^® technology, based on known chromatographic principles, outperforms HPLC in every respect.

HPLC Resolution

Optimized HPLC separation of acetaminophen, caffeine, 7-hydroxycoumarin and coumarin, USP resolution determined from acetaminophen and caffeine peaks.

UPLC Resolution

The ACQUITY UPLC System provides more resolution, while maintaining throughput.

Increased Speed and Sensitivity

The ACQUITY UPLC System provides increased throughput and sensitivity, while maintaining resolution.

HPLC Speed and Sensitivity

Optimized HPLC separation of acetaminophen, caffeine, 7-hydroxycoumarin and coumarin, USP resolution determined from acetaminophen and caffeine peaks.

UPLC Speed and Sensitivity

ACQUITY UPLC provides increased throughput and sensitivity, while maintaining resolution.

Increased Resolution, Speed, and Sensitivity

The ACQUITY UPLC System provides more resolution and sensitivity, while increasing throughput with optional elevated temperatures.

HPLC Resolution, Speed, and Sensitivity

Optimized HPLC separation of acetaminophen, caffeine, 7-hydroxycoumarin and coumarin, USP resolution determined from acetaminophen and caffeine peaks.

UPLC Resolution, Speed, and Sensitivity

ACQUITY UPLC provides more resolution and sensitivity, while increasing throughput with optional elevated temperatures.

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ACQUITY UPLC I-Class

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Select Science Silver Seal of Quality

The ACQUITY UPLC System was awarded a Silver Seal of Quality in recognition of it receiving 75 or more reviews from scientists globally.