MassLynx Skyline Interface

The MassLynx Skyline Interface provides a simple automated workflow for MRM method development for peptide & protein (digested) bioanalysis and targeted proteomics.

Skyline is freeware software, developed by the MacCoss Lab at the University of Washington, and the industry gold standard for building MRM methods for LC-MS/MS quantification of peptides and proteins. Skyline works with MassLynx MS software to create optimized highly selective and sensitive MRM methods, with minimal manual intervention, saving the user time & energy for confident routine analysis.

Learn more about our specifc products below:

• Waters MassLynx Software
• Skyline 

The MassLynx Skyline Interface can be freely downloaded from here.

For a full list of instruments supported please see the release notes.

MassLynx 4.2 installed on a Windows 10 or MassLynx 4.1 installed on a Windows 7 acquisition workstation.

The MassLynx Skyline Interface is an easy-to-use tool for automated optimization and fine tuning of MRM methods, enabling streamlined development of high-sensitivity assays, and is applicable for both biotherapeutics or biomarkers, including bioanalytical or targeted proteomics experiments.

• Identify unique peptides and optimize/fine-tune MRM transitions
  • MassLynx Skyline Interface
• Protein digestion and peptide level clean-up:
  • ProteinWorks Auto-eXpress Digest Kit
  • Hamilton STARWorks
• Data acquisition:
  • ACQUITY UPLC Peptide Columns
  • ACQUITY UPLC I-Class PLUS
  • Xevo TQ-XS
• Data processing and review results:
  • MassLynx/TargetLynx
  • Skyline

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