High pore volume HPLC particles do not possess the mechanical stability necessary to withstand the high pressures inherent in UPLC® separations. Waters material scientists addressed this challenge by developing a silica particle designed for high mechanical stability with the appropriate morphology to provide long UPLC column lifetimes and high UPLC efficiencies at high pressures. The 1.8 µm High Strength Silica (HSS) particle is the first and only 100% silica particle designed, tested and intended for use in applications up to 15,000 psi (1034 bar).
HSS particle technology is also available in HPLC particle sizes (3.5 and 5 µm) in the HSS family of HPLC columns, enabling seamless transfer between HPLC and UPLC technology platforms.
The ACQUITY UPLC® HSS C18 stationary phase features a tri-functionally bonded C18 ligand and proprietary endcapping process that yields superior peak shapes for bases at neutral pH. The HSS particle is 100% silica so the ACQUITY UPLC HSS C18 chemistry resists acid hydrolysis and provides extremely long lifetimes under demanding highly acidic conditions.
ACQUITY UPLC® HSS T3 Columns utilize Waters innovative and proprietary T3 bonding. T3 bonding utilizes a trifunctional C18 alkyl phase bonded at a ligand density that promotes polar compound retention and aqueous mobile-phase compatibility. The T3 endcapping is much more effective than traditional trimethyl silane (TMS) end-capping. This unique combination of bonding and endcapping also enhances column performance, lifetime, peak shape, loading capacity, method development, selectivity and stability.
Superior Polar Compound Retention
The ACQUITY UPLC HSS T3 Column is ideally suited for the enhanced retention of polar compounds and metabolites by reversed-phase LC. This low-ligand density C18 column enables analytes to more readily access the pore structure of the material, providing balanced retention of polar and hydrophobic molecules without the need for ion-pair reagents.
Method development scientists use selectivity and retentivity to influence chromatographic behavior. The selection of phases in the HSS Technology family offers the chromatographer the ability to retain highly polar analytes. Selectivity is manipulated through the use of pH, mobile-phase buffer strength, organic modifier and column choice.
UPLC to HPLC Method Transfer
The HSS Technology particles used in both ACQUITY UPLC columns and the HSS family of HPLC columns enables seamless methods transfer and the fastest route to an optimized LC method. HSS particles provide superior polar compound retention and enhance column performance, lifetime, peak shape and stability for both UPLC and HPLC systems.