In the arena of scientific discovery, the slow pace of advancement and escalating costs of research can prove to be a challenge. The SYNAPT XS mass spectrometer shatters these barriers by providing ultimate flexibility and greater freedom of analytical choice to support scientific creativity and technical success for any application.
Novel technology provides cutting edge performance
With technology building blocks inherited from the Waters Advanced Mass Spectrometry SELECT SERIES, advanced innovation is in-built, ensuring scientists using this platform are at the cutting edge of mass spectrometry whilst retaining the ease of use and established end to end workflows for which the SYNAPT is well known.
StepWaveTM XS - Redesigned segmented quadrupole transfer optics that provide enhanced sensitivity for challenging compounds while further improving the levels of analytical robustness.
Extended ToF - Delivers UPLC compatible mass resolution, matrix-tolerant dynamic range, and quantitative results for the most complex samples, whilst offering superior specifications to previous SYNAPT models.
With an ever-increasing need for the greatest variety of analytical strategies to effectively tackle inherently challenging questions, SYNAPT XS combines high performance with ultimate flexibility. Unlike competitors’ systems with restricted inlet options, scan function limitations or requiring multiple platforms, only Waters offers an all-encompassing high-performing LC-MS solution which, by design, provides greater freedom of analytical choice to support scientific discovery.
SONAR and HDMSE provide a unique toolkit for interrogating complex mixtures
A complete analytical strategy requires the combination of appropriate complementary techniques to see the bigger picture. With both SONAR and IMS based data independent acquisition (DIA) modes of operation on the SYNAPT XS, the discovery analyst is in a unique position to interrogate complex mixtures by complementary mechanisms. Both types of acquisition increase analytical peak capacity, providing ‘clean and clear’ fragmentation data, but based on different molecular properties. This provides a truly unique investigative toolbox for the in-depth interrogation of complex mixtures.
Find out more about the DIA methods available with the SYNAPT XS, and Waters’ decades of contributions to DIA innovation:
Ion Mobility and CCS measurement
Conventional mass spectrometers separate components on the basis of m/z. The SYNAPT XS also allows separation of molecules according to their size, shape, and charge during ion mobility experiments as a function of their collisional cross section (CCS). As well as Ion mobility offering an additional dimension of resolution, multiplying peak capacity and selectivity of analysis the measurement of CCS offers additional molecular identifiers. The measured CCS of an ion can be used to help confirm an ions identity or investigate its structure. The inclusion of ion mobility significantly increases the extent and confidence with which a scientist can profile complex mixtures and complex molecules and dramatically enhances sample definition.
CID and ETD Fragmentation Capabilities
The dual collision cell arrangement of TriWave enhances MS/MS possibilities, by providing Collision Induced Dissociation (CID) and/or Electron Transfer Dissociation (ETD) fragmentation with high resolution and accurate mass measurements. The resolving quadrupole is available in 4KDa, 8KDa or 32KDa mass range options for MS/MS of small to macromolecular species
Time aligned parallel (TAP) fragmentation is an acquisition mode unique to the T-Wave IMS design. It permits users to take the advantage of the TriWave configuration which allows the pre-IMS T-Wave and post-IMS T-Wave to operate as two separate collision cells. The resultant CID-IMS-CID instrumental operation helps to provide ultra confident structural characterization of components. TAP fragmentation provides a distinct advantage for building a complete structure, through superior fragment ion coverage, sensitivity, and accuracy compared to traditional MSn or MS/MS techniques.
As of 2020, Waters has over two decades of involvement in introducing, developing, and commercializing modes of data independent acquisition (DIA), culminating in the technologies deployed in the SYNAPT XS. Join us as we revisit the advances in DIA.
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With its ease-of-use, flexibility and speed, CCS prediction is a highly useful tool to provide reference and guidance for qualitative analysis.
Dr. Gaétan Glauser, a senior scientist at Neuchâtel found the SYNAPT XS’s versatility and high performance to be a perfect match for the facility’s research.
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