DESI™ XS has been designed to deliver improved build quality, reliability and simplicity with innovation in mind.
The source is deployable across the Waters HRMS portfolio, bringing to the market a flexible solution and ensuring a consistent, high-level of performance to all imaging customers.
DESI XS is a foundation upon which the next generation of developments in Waters DESI MS imaging will be built, future proofing MS imaging needs.
DESI MS imaging source
Desorption Electrospray Ionization (DESI) mass spectrometry is a technique that allows chemical information to be obtained directly from a wide range of surfaces. DESI imaging provides information on the spatial distribution of molecules at or near the surface. DESI provides beautiful and information-rich images that allow scientists to answer the fundamental question: "Where is this component in my sample?"
Determine the spatial localization of the components of samples more quickly and easily with both increased sensitivity and clarity using the high-performance sprayer. This is complemented by more efficient ion transfer with the heated transfer line, further enhancing the sensitivity and molecular range of the DESI technique, making it possible to visualize compounds at previously unobtainable levels.
Improvements in sprayer usability and reproducibility allow higher quality data acquisitions with minimum optimization, enabling the translation of compound visualization into new knowledge and applied action quickly and easily.
These combined technologies contribute to improvements in overall in lab productivity, accelerating the rate of image generation by limiting the time required to acquire data at each individual pixel point.
Figure 3 shows a DESI XS generated image of murine brain section, demonstrating the spatial distribution of three different lipids (red, green and blue). Different spatial distributions within the brain suggest they serve different molecular functions.
In Figure 4, A. displays DESI images of [M-H]- lipid species in mouse kidney tissue sections, showing 20 and 40 µm spatial resolution. In B., it is possible to see a zoomed-in view of the same region of the kidney.
Major milestones in DESI
For more than a decade, Waters™ has been actively involved in the MS Imaging technology space, with a focus on DESI imaging for the past six years. In order to build upon the continuing success in MS imaging, as of October 1, 2019, Waters acquired exclusive rights to DESI technology from Prosolia Inc. and the Purdue Research Foundation. With commitment to DESI technology complementing existing MALDI technology, Waters is positioned to pursue an exciting future advancing the field of MS imaging.
The source design includes a partially sealed housing that increases environmental stability and reduces atmospheric interference during the sample analysis, compared to an open DESI source. It offers a secure environment for both the user and the sample, providing a potential barrier from solvent vapors and sample aerosols while protecting the sample under analysis from contamination.
Unlike previous top-down visualization, the source also provides a camera for viewing the side of the DESI sprayer and the sample. This helps the user to locate the optimal spraying position for the DESI analysis more easily.
Internal components of the source have been designed with robustness and usability in mind. The sprayer armature is precision manufactured with a spring loaded arm encompassing caged roller bearings to give precision and durability for large scale acquisitions.
Partnering with a leading microscope stage manufacturer, a cutting-edge stepper motor stage has been incorporated into the source which has been designed to utilize different holders, enabling examination of a wide range of specimen types. Its unique S curve acceleration algorithm allows fast, smooth positioning without vibration, reducing disruption to samples and promising focused, consistent imaging.
For the first time, the source boasts integrated electronics and gas handling, meaning gases and voltages are automatically shut off at the end of your analysis. This is not only more environmentally friendly, but also saves on the cost of MS imaging consumables and prolongs the life of the DESI sprayer.
Accelerated discovery through enhanced information
The DESI XS source enables the generation of ion images, with consistent, reliable signal intensity allowing for clear visualization of molecular spatial distributions. Discovery research is accelerated through greater confidence in decision making during analyses. This source ensures images that display clearly defined distributions and components, and are easily and rapidly processed through High Definition Imaging (HDI™) Software.
Additionally, DESI XS is a core component of the Full Spectrum Molecular Imaging system solution uniquely available on the SYNAPT™ XS and SELECT SERIES™ MRT. This solution utilizes a combination of analytical techniques which are well suited to analyze peptides, lipids and small molecule metabolites. The dual ionization techniques employed complement each other to deliver the most comprehensive level of information possible with MS Imaging.
Futureproofing laboratories’ evolving imaging needs with Waters’ next-generation DESI MS imaging solutions.
The DESI XS source gives access to a minimal sample preparation method to answer easily, quickly, and accurately, “where is this component in my sample?”.
Access scalable imaging across the Waters MS portfolio, including the SELECT SERIES™ MRT, SELECT SERIES™ Cyclic IMS, SYNAPT™ XS, and Xevo™ G2-XS QToF.
Accelerate your research and comprehensively identify analytes in your complex samples with the SELECT SERIES MRT, built with state-of-the-art Multi Reflecting Time-of-Flight (MRT) technology.
The SYNAPT XS mass spectrometer shatters the barriers of scientific discovery by providing ultimate flexibility and greater freedom of analytical choice, to support technical success for any application.
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The SELECT SERIES Cyclic IMS combines novel cyclic ion mobility separation with state-of-the-art high performance and Tof MS, enabling leading researchers to unlock the potential in scientific discovery.
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