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This course is designed to help individuals gain a solid understanding of method development and validation procedures used to confirm proper chromatographic technique. The relationship between method development and method validation and how this relationship can be used to meet chromatographic goals is discussed. Attendees will learn proven troubleshooting techniques for identifying and differentiating chromatographic issues involving chemistry versus instrumentation.
*DECEMBER 14 - 17, 2020. This two day training will be held virtually on Tuesday, December 14 to Friday, December 17 from 11:00 AM EDT to 3:00 PM EDT each day.
Virtual Classroom – In the virtual classroom experience each user will be invited into a Microsoft Teams Meeting and ReadyTech Event session. In the Teams Meeting the students will be able to speak with the instructor and other students and watch as the instructor provides live demonstrations. Invitations will be sent prior to the start of the class. An internet connection and a computer system are required for this course. The Teams and ReadyTech platforms are accessed through internet browser windows.
An Electronic course manual is included in the price of course. Hard Copy course manuals are available for an additional charge. See course notes for details.
- Basic theory required in systematically optimizing HPLC separations
- Basic theory underlying reversed-phase columns and reversed-phase chromatography
- Techniques required for sample quantitation and method validation
- Developing and using gradient elution separations
- Troubleshooting separations for chemistry versus instrument issues
Day 1 Includes:
- Introduction to HPLC Theory
- Reversed-Phase Chromatography Theory & Practice
- Gradient Elution Chromatography
Day 2 Includes:
- HPLC Method Validation using ICH Guidelines
- Sample Preparation for Complex Samples
- Instrumental considerations for obtaining valid results
This course is designed for lab personnel who are responsible for operating HPLC systems and/or developing HPLC separations.
Attendees should have beginner through intermediate level HPLC experience. Some exposure to the chemistry equivalent of general chemistry and introductory organic chemistry is helpful
Each attendee receives an electronic course manual for reference and a Certificate of Training
If you would like to receive a hard copy of the course manual, enter “HCM” under comments on your registration form. There will be an additional fee of $69.00.
Call 1-800-252-4752, ext 8704. For MS Technology call 1-800-252-4752, ext.
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