• Application Note

ACQUITY UPLC Analysis of Water Soluble Vitamins

ACQUITY UPLC Analysis of Water Soluble Vitamins

  • Waters Corporation

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates the UPLC Analysis of water soluble vitamins.

Introduction

Structures

Compounds

1. Nicotinamide (25 μg/mL)

2. Pyridoxal (50 μg/mL)

3. Riboflavin (50 μg/mL)

4. Nicotinic acid (50 μg/mL)

5. Thiamine (50 μg/mL)

6. Ascorbic acid (25 μg/mL)

7. B12 (50 μg/mL)

8. Folic Acid (25 μg/mL)

Experimental

Test conditions

Column:

ACQUITY UPLC BEH Amide, 2.1 x 50 mm, 1.7 μm

Part Number:

186004800

Mobile Phase A:

50/50 MeCN/H2O with 10 mM CH3COONH4 and 0.04 % NH4OH, pH 9.0

Mobile Phase B:

90/10 MeCN/H2O with 10 mM CH3COONH4 and 0.04 % NH4OH, pH 9.0

Flow Rate:

0.5 mL/min

Injection Volume:

5 μL (PLNO)

Sample Diluent:

75/25 MeCN/MeOH with 0.2% HCOOH

Column Temperature:

30 °C

Weak Needle Wash:

95/5 MeCN/H2O

Detection:

UV @ 265nm

Sampling Rate:

20 points/sec

Filter Time Constant:

0.2

Instrument:

Waters ACQUITY UPLC with ACQUITY UPLC PDA Detector

Gradient:

Time (min)

%A

%B

Initial

0.1

99.9

3.50

70.0

30.0

3.51

0.1

99.9

7.50

0.1

99.9

Results and Discussion

WA60093, June 2009

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